Atomic Absorption Spectrophotometry

Analysis Steps

1.    Find the page on instrument conditions in the Perkin Elmer manual for
       the element you are running.   The following elements can be found
      by clicking on their link:  Ca, Mg, K, Na.

2.   Note the linear working range for the analyte.  Make up a set of standards
     using the linear working range as a guideline.  See making standards for
     Ca/Mg  or    K/Na

3.   Add reagents to correct for interference:
            (CsCl for Na and K;  LaCl₃ for Ca and Mg)

4.   Find the key to the Mechanical Equipment room in the small drawer
      under the AA.  The room is directly across from 028.   You must have
      a staff member with you when you enter this room.   Close the valve on
      the bottom of the tank by turning clockwise.   Push yellow switch on top
      to the rear to start the compressor.   Verify that the pressure goes up and
      that the air drain is not leaking.   The pressure should reach 40 psi before
      the compressor turns off.  Make sure the room is locked when you leave.

4.   Find appropriate hollow cathode lamp.  Look for and note continuous
      current reading on side of lamp.

5.   Install the lamp in the lamp turret.  Push it in to within 1/4" of the
      metal cabinet.  Plug the lamp into the HCL socket in the back of the
      lamp compartment.

6.   Switch the POWER to ON and let the computer boot up.  The AA starts in
      the "Element Select" screen.   You can get back to this screen at any time
      by pressing this button on the upper left of the key pad.

7.   Enter the atomic number of your analyte from the list on the screen.

8.   Examine the methods that are displayed, which use different wavelengths.
      Usually the first one is the most sensitive and is preferred.  Push the
      appropriate soft key (under the screen) to select that method.  This will
      shift you to the "Program Mode" screen (the Prog button).

9.