Introduction and UV-Vis Spectrophotometry

Instrumentation

Electromagnetic Spectrum

Nature of Light

Molecular absorption

Spec. Cell Beer's Law

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Instrumentation used to quantify environmental analytes

        1.  Spectrophotometric Techniquesbased on light absorption
            a.  UV/Vis Spectrophotometry 
(phosphate, silica)
             b.  Atomic Absorption Spectrophotometry  -
(metals)
             c.  Infra-red gas analysis  
-   (TOC)

        2.
Chromatographic techniques  -  (separations)
            a. High Performance Liquid Chromatography 

                                (polar organics - many pesticides) 

                 1. Ion chromatography (anions)
            b. Gas chromatography
(nonpolar organics - chlorinated hydrocarbons)
 
        3.
Potentiometric techniquesAnalyte forms electrical circuit w/ sensor
            a. 
Ion-selective electrodes - pH, DO, F-, etc.
   
              b.  Potentiometric Titrations  - Alkalinity

        4.
Mass spectrometry  - often as detector coupled to others (e.g. GC/MS)
   
                       molecule broken up into characteristic fragments