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Introduction and UV-Vis Spectrophotometry |
| Spec. Cell | Beer's Law |
Instrumentation used to quantify environmental analytes
1.
Spectrophotometric
Techniques - based
on light absorption
a. UV/Vis Spectrophotometry
- (phosphate,
silica)
b. Atomic
Absorption Spectrophotometry - (metals)
c.
Infra-red gas analysis
- (TOC)
2. Chromatographic techniques
- (separations)
a. High
Performance Liquid Chromatography
(polar organics - many pesticides)
1. Ion chromatography (anions)
b. Gas chromatography
(nonpolar organics - chlorinated hydrocarbons)
3. Potentiometric techniques
- Analyte forms
electrical circuit
w/ sensor
a. Ion-selective electrodes
- pH, DO, F-, etc.
b. Potentiometric
Titrations - Alkalinity
4. Mass spectrometry
-
often as detector coupled to others (e.g.
GC/MS)
molecule broken up
into characteristic fragments